The Laboratory for Centrosome and Cytoskeleton Biology  

Original Publications from the lab  (Peer reviewed work papers, Selected)

• Gabriel, E., et al. Gopalakrishnan J*. Nature Protocols. 2023 May 17. doi: 10.1038/s41596-023-00814-x.
• Sudhakar DVS, et al. Gopalakrishnan J*, Thangaraj K*. Hum Mol Genet. 2022 Sep 1:ddac216. doi: 10.1093/hmg/ddac216.
• Gabriel, E., et al. Gopalakrishnan J*. Cell Stem Cell. 2021 Aug 12:S1934-5909(21)00295-2. doi: 10.1016/j.stem.2021.07.010.
• Goranci-Buzhala, G., et al. Gopalakrishnan J*. Cell Reports. 2021 Sep 7;36(10):109656. doi: 10.1016/j.celrep.2021.109656.
• Anand Ramani, et al. Gopalakrishnan J*.  EMBO Journal. 2020 Oct 15;39(20):e106230. doi: 10.15252/embj.2020106230. (Cover article)
• Goranci-Buzhala G, et al. Gopalakrishnan J*. Cell Rep. 2020. Jun 9;31(10):107738. doi: 10.1016/j.celrep.2020.107738
• Arul Mariappan et al. Gopalakrishnan J*.  EMBO Journal  2019. Dec 10. pii: e99876. doi: 10.15252/embj.201899876
• Ramani A,  et al. Gopalakrishnan J*. Cell Reports,  2018. Dec 26;25(13):3618-3630.e6
• Gabriel E, et al. Gopalakrishnan J*. Cell Stem Cell 20, 2017. 1-10.
• Zheng X, et al. Gopalakrishnan J*, Li H*. Nat Commun,  7, 2016. 11874.
• Gabriel E, et al. Gopalakrishnan J*.  EMBO Journal. 2016. Apr 15;35(8):803-19 (Cover article)
• Zheng X, et al. Gopalakrishnan J*. PNAS, 2014. Volume 111, Number 3.
• Gopalakrishnan J, et al. Avidor-Reiss T*. Nat Cell Biol 14, 865-873.
• Gopalakrishnan J, et al. Avidor-Reiss T*. Nat Commun. 2011. 2, 359.
• Gopalakrishnan J, et al. Avidor-Reiss T*. Journal of Biological Chemistry. 2010. 285, 8759-8770.

 

All Publications (Original papers from the lab, collaborations, reviews, and commentaries)

2023:

• Gopalakrishnan J, Feistel K, Friedrich BM, Grapin-Botton A, Jurisch-Yaksi N, Mass E, Mick DU, Müller RU, May-Simera H, Schermer B, Schmidts M, Walentek P, Wachten D. Emerging principles of primary cilia dynamics in controlling tissue organization and function. EMBO J. 2023 Sep 25:e113891. doi: 10.15252/embj.2023113891.
• Gabriel E, Albanna W, Pasquini G, Ramani A, Josipovic N, Mariappan A, Riparbelli MG, Callaini G, Karch CM, Goureau O, Papantonis A, Busskamp V, Schneider T, Gopalakrishnan J. Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia.
  Nat Protoc. 2023 May 17. doi: 10.1038/s41596-023-00814-x.
• Chandrasegaran P, Nabilla Lestari A, Sinton MC, Gopalakrishnan J, Quintana JF. Modelling host- Trypanosoma brucei gambiense interactions in vitro using human induced pluripotent stem cell-derived cortical brain organoids. F1000Res. 2023 Jul 28;12:437. doi: 10.12688/f1000research.131507.2. eCollection 2023.
•  Bieder A, Chandrasekar G, Wason A, Erkelenz S, Gopalakrishnan J, Kere J, Tapia-Páez I.  Genetic and protein interaction studies between the ciliary dyslexia candidate genes DYX1C1 and DCDC2. BMC Mol Cell Biol. 2023 May 26;24(1):20. doi: 10.1186/s12860-023-00483-4.
• Altinisik N, Rathinam D, Tran M, Gopalakrishnan J. Brain organoids restore cortical damage. Cell Stem Cell. 2023 Mar 2;30(3):241-242. doi: 10.1016/j.stem.2023.01.012. Epub 2023 Feb 9.
• Lee D, Gimple RC, Wu X, Prager BC, Qiu Z, Wu Q, Daggubati V, Mariappan A, Gopalakrishnan J, Sarkisian MR, Raleigh DR, Rich JN. J Superenhancer activation of KLHDC8A drives glioma ciliation and hedgehog signaling. Clin Invest. 2023 Jan 17;133(2):e163592. doi: 10.1172/JCI163592.

• Ceruti T, D'Alessandris QG, Frapolli R, Gopalakrishnan J, Buccarelli M, Meroni M, Lauretti L, Ricci-Vitiani L, Pallini R, Zucchetti M. Development and Validation of a HPLC-MS/MS Method to Measure Nifuroxazide and Its Application in Healthy and Glioblastoma-Bearing Mice. Pharmaceutics. 2022 Sep 28;14(10):2071. doi: 10.3390/pharmaceutics14102071.
• Sudhakar DVS, Phanindranath R, Jaishankar S, Ramani A, Kalamkar KP, Kumar U, Pawar AD, Dada R, Singh R, Gupta NJ, Deenadayal M, Tolani AD, Sharma Y, Anand A, Gopalakrishnan J*, Thangaraj K. Exome sequencing and functional analyses revealed CETN1 variants leads to impaired cell division and male fertility. Hum Mol Genet. 2022 Sep 1:ddac216. doi: 10.1093/hmg/ddac216.
• Stein A, Hilken Née Thomopoulou P, Frias C, Hopff SM, Varela P, Wilke N, Mariappan A, Neudörfl JM, Fedorov AY, Gopalakrishnan J, Gigant B, Prokop A, Schmalz HG. B-nor-methylene Colchicinoid PT-100 Selectively Induces Apoptosis in Multidrug-Resistant Human Cancer Cells via an Intrinsic Pathway in a Caspase-Independent Manner. ACS Omega. 2022 Jan 11;7(3):2591-2603. doi: 10.1021/acsomega.1c04659. eCollection 2022 Jan 25. PMID: 35097257
• Karadag C, Gopalakrishnan J, von Saß C, Cornelius JF, Hänggi D, van Lieshout JH, Kamp MA. Non-Animal Models in Experimental Subarachnoid Hemorrhage Research: Potentials and the Dilemma of the Translation from Bench to Bedside. Transl Stroke Res. 2022 Apr;13(2):218-221. doi: 10.1007/s12975-021-00950-0. Epub 2021 Oct 29.

 

2021:

• Gabriel, E., Albanna, W., Pasquini, G., Ramani, A., Josipovic, N., Mariappan, A., Schinzel, F., Karch, C.M., Bao, G., Gottardo, M., et al. Gopalakrishnan J* (2021). Human brain organoids assemble functionally integrated bilateral optic vesicles. Cell Stem Cell. 2021 Aug 12:S1934-5909(21)00295-2. doi: 10.1016/j.stem.2021.07.010.

  • ​Received international attention in more than 200 news outlets, Use the search term "Mini brains with eye-like structures".

  • See highlight: The eyeballs connected to the brain ball by Rajesh C Rao, Jeffery H Stern, and Sally Temple. Cell Stem Cell.  DOI:   10.1016/j.stem.2021.09.010

• Goranci-Buzhala, G., Mariappan, A., Ricci-Vitiani, L., Josipovic, N., Pacion, S., Gottardo, M., Ptok, J., Callaini, G., Rajalingam, K., Dynlacht, B., et al. Gopalakrishnan J*.Cilium induction triggers the differentiation of glioma stem cells. Cell Reports. Sep 7;36(10):109656. doi: 10.1016/j.celrep.2021.109656.

• Ramani A, Pranty AI, Gopalakrishnan J*. Neurotropic effects of SARS-CoV-2 modeled by the Human Brain Organoids. Stem Cell Reports. 2021 Mar 9;16(3):373-384.

 

2020:

• Mariappan A, Goranci-Buzhala G, Ricci-Vitiani L, Pallini R , Gopalakrishnan J*. Trends and challenges in modeling glioma using 3D human brain organoids. Cell Death Differ. 2020 Dec 1:1-9. doi: 10.1038/s41418-020-00679-7.
• Farooq M [...] Gopalakrishnan J [..] Christensen ST, Larsen LA. RRP7A links primary microcephaly to dysfunction of ribosome biogenesis, resorption of primary cilia, and neurogenesis. Nat Commun. 2020 Nov 16;11(1):5816. doi: 10.1038/s41467-020-19658-0.
• Anand Ramani, Lisa Muller, Philipp Niklas Ostermann [...] Heiner Schaal* and Gopalakrishnan J*. SARS-CoV-2 targets neurons of 3D human brain organoids. EMBO Journal. 2020 Oct 15;39(20):e106230. doi: 10.15252/embj.2020106230. (Cover article)
• Goranci-Buzhala G, Mariappan A, Gabriel E, Ramani A, Ricci-Vitiani L, Buccarelli M, D'Alessandris QG, Pallini R, Gopalakrishnan J*. Rapid and efficient invasion assay of glioblastoma in human brain organoids. Cell Rep. 2020. Jun 9;31(10):107738. doi: 10.1016/j.celrep.2020.107738
• Gabriel E, Ramani A, Altinisik N, Gopalakrishnan J*. Human brain organoids to decode mechanisms of microcephaly. Front Cell Neurosci. 2020 May 8;14:115. doi: 10.3389/fncel.2020.00115. eCollection 2020.
• Liu W et al, Gopalakrishnan J and Pandyra AA*. Repurposing the serotonin agonist Tegaserod as an anticancer agent in melanoma: Molecular mechanisms and clinical implications. J Exp Clin Cancer Res. 2020 Feb 21;39(1):38.

 

2019:

• Lapart JA et al, Gopalakrishnan J and Durand B*. Dzip1 and Fam92 form a ciliary transition zone complex with cell-type-specific roles in Drosophila.  Elife. 2019 Dec 10;8 pii: e49307
• Gopalakrishnan J*. The Emergence of Stem Cell-Based Brain Organoids: Trends and Challenges.  Bioessays. 2019 Aug;41(8):e1900011. doi: 10.1002/bies.201900011.

• Arul Mariappan Soni K, Schorpp K, Zhao F, Minakar A, Zheng X, Mandad S, Macheleidt I, Ramani A, Kubelka T, Dawidowski M, Golfmann K, Wason A, Yang C, Simons J, Schmalz HG, Hyman AA, Aneja R, Ullrich R, Urlaub H, Odenthal M, Büttner R, Li H, Sattler M, Hadian K, Gopalakrishnan J*. Inhibition of CPAP-Tubulin interaction prevents the proliferation of centrosome amplified cancer cells. EMBO Journal  2019. Dec 10. pii: e99876. doi: 10.15252/embj.201899876

  • Highlighted in EMBO J as Magic Bullet

 

2018:

• Ramani A, Mariappan A, Gottardo M, Mandad S, Urlaub H, Avidor-Reiss T, Riparbelli M, Callaini G, Debec A, Feederle R,et al., Gopalakrishnan J*. Plk1/Polo phosphorylates Sas-4 at the onset of mitosis for an efficient recruitment of pericentriolar material to centrosomes. Cell Reports,  2018. Dec 26;25(13):3618-3630.e6 (2018).
• Lilian A Martinez Carrera, Elke Gabriel, Colin Donohoe, Irmgard Hölker, Aruljothi Mariappan, Markus Storbeck, Mirka Uhlirova, Jay Gopalakrishnan, Brunhilde Wirth* Novel insights into SMALED2: BICD2 mutations increase microtubule stability and cause defects in axonal and NMJ development.  Human Molecular Genetics, 2018. May 15;27(10):1772-1784

 

2017:

• Goranci-Buzhala G, Gabriel E, Mariappan A, Gopalakrishnan J* Losers of Primary Cilia Gain the Benefit of survival. Cancer Discovery 12, 2017. 1374-1375.
• Gabriel E, Gopalakrishnan J* Generation of iPSC-derived human brain organoids to model early neurodevelopmental disorders. J Vis Exp. 14; 2017. (122).
• Gabriel E, Ramani A, Karow U, Gottardo M, Natarajan K, Gooi LM, Goranci-Buzhala G, Krut O, Peters F, Nikolic M, Kuivanen S, Korhonen E, Smura T, Vapalahti O, Papantonis A, Schmidt-Chanasit J, Riparbelli M, Callaini G, Krönke M, Utermöhlen O, Gopalakrishnan J*. Recent Zika virus isolates induce premature differentiation of neural progenitors in human brain organoids. Cell Stem Cell 20, 2017. 1-10.

 

2016:

• Li Ming Gooi and Gopalakrishnan J* Regulation of stem cells in their niche. Current Stem Cell Reports, 3, 3, 2016. 282-289.
• Zheng X, Ramani A, Soni K, Gottardo M, Zheng S, Ming Gooi L, Li W, Feng S, Mariappan A, Wason A, Widlund P, Pozniakovsky A, Poser I, Deng H, Ou G, Riparbelli M, Giuliano C, Hyman AA, Sattler M, Gopalakrishnan J*, Li H*. Molecular basis for CPAP-tubulin interaction in controlling centriolar and ciliary length. Nat Commun 7, 2016. 11874.
• Gabriel E, Wason A, Ramani A, Gooi LM, Keller P, Pozniakovsky A, Poser I, Noack F, Telugu NS, Calegari F, Šarić T, Hescheler J, Hyman AA, Gottardo M, Callaini G, Alkuraya FS, Gopalakrishnan J*. CPAP promotes timely cilium disassembly to maintain neural progenitor pool.  EMBO Journal. 2016. Apr 15;35(8):803-19. (Cover article)
• Wike CL et al., Jay Gopalakrishnan and Tyler JK*. Excess-free histone H3 localizes to centrosomes for proteasome-mediated degradation during mitosis in metazoans. Cell Cycle. 2016 Jun 1:0.
• Brenke et al., and Jay Gopalakrishnan, Hadian K* Identification of Small Molecule Frequent Hitters of GST–Glutathione interaction. J Biomol Screen. 6 Apr 4. 2016.

 

2015:

• Persefoni Thomopoulou et al., Gopalakrishnan J and Hans-Günther Schmalz*. New Colchicine-Derived Triazoles and Their Influence on Cytotoxicity and Microtubule Morphology. ACS Med. Chem. Lett. ACS Med Chem Lett. 2015. Dec 29;7(2):188-91.
• Anand Ramani, Li Ming Gooi and Gopalakrishnan J*. Building the meat of pericentriolar material surrounding the skeleton of a centriole. Cell News 2/2015, German Society for Cell Biology (DGZ)

 

2014:

• Zheng X, Gooi LM, Wason A, Gabriel E, Mehrjardi NZ, Yang Q, Zhang X, Debec A, Basiri ML, Avidor-Reiss T, Pozniakovsky A, Poser I, Saric T, Hyman AA, Li H*, Gopalakrishnan J* Conserved TCP  domain of Sas-4/CPAP is essential for pericentriolar material tethering during centrosome biogenesis. PNAS, 2014. Volume 111, Number 3.
• Pannu V, Rida PC, Celik B, Turaga RC, Ogden A, Cantuaria G, Jay Gopalakrishnan, Aneja R*. Centrosome-declustering drugs mediate a two-pronged attack on interphase and mitosis in supercentrosomal cancer cells.  Cell Death Dis. 2014. Nov 20;5:e1538. doi: 10.1038/cddis.2014.505.

 

2013:

• Avidor-Reiss T, and Jay Gopalakrishnan. Cell Cycle Regulation of the Centrosome and Cilium.  Drug Discov Today Dis Mech. 2013. Dec 1;10(3-4):e119-e124
• Avidor-Reiss T, and Gopalakrishnan J Building a centriole. Curr Opin Cell Biol; 2013. S0955-0674(12)00180-9.
• Schorpp K, Rothenaigner I, Salmina E, Reinshagen J, Low T, Brenke JK, Gopalakrishnan J, Tetko IV, Gul S, Hadian K*. Identification of Small-Molecule Frequent Hitters from AlphaScreen High-Throughput Screens. J Biomol Screen. Dec 26, (2013).

 

2012:

• Gopalakrishnan J, Chim YC, Ha A, Basiri ML, Lerit DA, Rusan NM, and Avidor-Reiss T. Tubulin nucleotide status controls Sas-4-dependent pericentriolar material recruitment. Nat Cell Biol 14, 865-873.

 

2011:

• Gopalakrishnan J, Mennella V, Blachon S, Zhai B, Smith AH, Megraw TL, Nicastro D, Gygi SP, Agard DA, and Avidor-Reiss T . Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome. Nat Commun. 2011. 2, 359.

 

2010:

• Gopalakrishnan J, Guichard P, Smith AH, Schwarz H, Agard DA, Marco S, and Avidor-Reiss T. Self-assembling SAS-6 Multimer Is a Core Centriole Building Block. Journal of Biological Chemistry. 2010. 285, 8759-8770.

 

2008:

• Blachon S, Gopalakrishnan J, Omori Y, Polyanovsky A, Church A, Nicastro D, Malicki J, and Avidor-Reiss T (2008). Drosophila asterless and Vertebrate Cep152 Are Orthologs Essential for Centriole Duplication. Genetics. 2008. 180, 2081-2094.

 

​2004:

• Gopalakrishnan J, Görisch H, Adrian L. Studies on hydrogenase activity and chlorobenzene respiratio in Dehalococcoides sp. strain CBDB1. Arch Microbiol. (2004) Dec;182(6):498-504.
• Gopalakrishnan J, Görisch H, Adrian L. Dehalorespiration with hexachlorobenzene and pentachlorobenzene by Dehalococcoides sp. strain CBDB1. Arch Microbiol. (2003) Dec;180(6):411-6.

 

 

Book chapters:

• T Avidor-Reiss, J Gopalakrishnan, ML Basiri. Sas-4-mediated PCM assembly is regulated by Tubulin Dimers. MOLECULAR BIOLOGY OF THE CELL 23. (2012)

 

• G Ben-David, J Gopalakrishnan, T Avidor-Reiss. SAS-6 Tetramers Self-assemble in the Formation of the Centriole Central Tubule in Drosophila IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 47, S43-S44 (2011)

 

• J Gopalakrishnan, YC Chim, A Ha, M Basiri, T Avidor-Reiss. A Sas-4/tubulin module regulates the formation of centrosomal complexes and centrosome biogenesis. MOLECULAR BIOLOGY OF THE CELL 22 (2011).

 

• T Avidor-Reiss, J Gopalakrishnan, S Blachon, A Polyanovsky. Centriole duplication and inheritance in Drosophila melanogaster. The Centrosome, 3-31 6 (2012).